Dental infection is being considered as one of the six most widespread non-communicable diseases throughout the world. Berberis aquifolium have shown positive antimicrobial, antifungal and anti-inflammatory actions in several microbial studies. So, this study was conducted to determine the antimicrobial efficacy of B. aquifolium against dental biofilm forming Streptococcus mutans and common oral pathogens.
The dental caries samples were collected from 5 patients of age group 7-35yrs from Kottayam Dist., Kerala, under aseptic condition. All the collected plate samples were cultured on nutrient, blood and Mitis Salivary Agar plates and colonies were counted. Microbial species were identified on the basis of morphological and biochemical studies. Microbiological assay (agar well diffusion method and minimal inhibitory concentration (MIC) to determine inhibition zone against oral pathogens were performed. Thus this study concluded that the ethanolic extracts of B. aquifolium showed significant MIC against S. mutans, Candida albicans and Pseudomonas aeruginosa compared to that of standard drug.
Inhaltsverzeichnis (Table of Contents)
- 1. Introduction
- 1.1. Objective of the Study
- 2. Review of Literature
- 2.1 Teeth and its structure
- 2.2 Pathogenicity of dental infection
- 2.3 Dental caries
- 2.4 Microbial etiology of caries
- 2.5 Treatment of caries
- 3. Materials and Methods
- 3.1 Chemicals
- 3.2 Preparation of powdered form of Homeopathic mother tinctures (HMT)
- 3.3 Collection of dental caries samples
- 3.4 Isolation and screening of bacteria
- 3.5 Morphological and biochemical identification of isolates
- 3.6 Identification of Streptococcal Isolates
- 3.7 Physical characterization
- 3.8 Biochemical characterization
- 3.9 Anti-microbial screening of extracts
- 3.10 MIC determination
- 4. Results
- 4.1 Isolation of bacteria from plaque samples based on their colony morphology
- 4.2 Biochemical identification of Colony 2
- 4.3 Biochemical identification of Colony 8
- 4.4 Biochemical identification of Colony 9
- 4.5 Identification of Colony 1 (budding Yeast cells) by Germ tube assay
- 4.6 Screening of DMSO (Negative control) and standard drugs (Positive control)
- 4.7 Inhibitory effects of B. aquifolium against dental biofilm producers and normal dental
- 4.8 Minimum inhibitory concentration (MIC) of B. aquifolium against dental pathogens
- 5. Discussion
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This study investigates the antibacterial properties of the homeopathic mother tincture of Berberis aquifolium against biofilm-forming bacteria associated with dental plaque. The objective is to assess the effectiveness of this herbal remedy in controlling dental infections.
- Antimicrobial effects of Berberis aquifolium
- Dental biofilm formation and its role in dental infections
- Identification and characterization of bacteria associated with dental plaque
- Evaluation of the minimum inhibitory concentration (MIC) of Berberis aquifolium
- Exploring the potential of herbal remedies for dental health
Zusammenfassung der Kapitel (Chapter Summaries)
The study begins with an introduction outlining the prevalence of dental infections and the limitations of conventional treatment options. Chapter 2 provides a comprehensive review of the literature focusing on the anatomy of teeth, the pathogenesis of dental infections, and the microbial etiology of caries. It also discusses the current treatment modalities for caries. Chapter 3 details the materials and methods used in the study, covering aspects such as the preparation of the homeopathic mother tincture, the collection of dental plaque samples, and the isolation and characterization of bacteria.
Chapter 4 presents the results obtained from the study, focusing on the isolation and identification of bacteria from dental plaque samples, the determination of their biochemical properties, and the assessment of the antibacterial activity of Berberis aquifolium. The chapter discusses the MIC values of the herbal remedy against various dental pathogens. While the final chapter summarizing the discussion and conclusions of the study is excluded, Chapter 5 provides a detailed analysis of the findings, emphasizing the efficacy of Berberis aquifolium as an antimicrobial agent against dental biofilm-forming bacteria.
Schlüsselwörter (Keywords)
The primary focus of this study lies in the antibacterial properties of Berberis aquifolium against dental plaque, specifically its effect on biofilm-forming bacteria. Key terms include dental caries, microbial etiology, biofilm formation, homeopathic mother tincture, minimum inhibitory concentration (MIC), antimicrobial activity, and herbal remedies for dental health.
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- Dr RATHEESH MOHANAN (Author), Dr Nirmal Ghosh (Author), Sulumol Thomas (Author), Sheethal Sreevallabhan (Author), Svenia Jose (Author), Asha Sukumarapillai (Author), Parvathy UJ (Author), Dr Sandya Sukumaran (Author), Sony Rajan (Author), 2021, Antibacterial Effect of Berberis Aquifolium Homeopathic Mother Tincture against Biofilm Formers from Dental Plaque, Munich, GRIN Verlag, https://www.hausarbeiten.de/document/986994
