The present study focuses on medicinal legumes such as Atylosia albicans (Wight & Arn.)Benth, Caesalpinia mimosoides (Lam), Derris scandens (Benth), Humboldtia brunonis (Wall), Indigofera cassioides (Roxb), Kingiodendron pinnatum (DC), and Tephrosia tinctoria (Baker), belonging to the family Fabaceae. These legumes were collected from different biogeographical regions of Western Ghats of Karnataka. Herbaria of these plants with taxonomic details are deposited in Biodiversity laboratory, Department of Environmental Science, University of Mysore, Mysore, Karnataka, India. Phytochemical screening, antimicrobial of these plants was carried out.
In the present investigation, bioautography method is used for the identification of bioactive molecule from the ethyl acetate extract of Kingiodendron pinnatum. This technique has been used for the detection of antimicrobial substances in many biological extracts. This method eliminates the need for the development of large numbers of plates in multiple solvent systems, reduces the amount of waste solvents for disposal, and substantially reduces the time needed to identify active compounds.
The results indicated that the isolated compound, 2-formyl-12-oxo-A-norcleroda-2, 13(16)-dien- 15-oic acid is a diterpene. The database search on phytochemicals present in the Kingiodendron pinnatum reported by earlier workers did not record the presence and biological activity of this diterpene, which is well described in the present study. This diterpene is a novel chemical entity described first time from the ethyl acetate extract of the plant Kingiodendron pinnatum. The study carried out on the seven legumes have clearly depicted their potential medicinal value. It is therefore necessary to give prime importance for their conservation. In view of this conservation of medicinal plants by using Ex-situ method was made for sustainable development. Of all the plants tested for seed germination and survival status Kingiodendron pinnatum showed the highest percentage of survival and acclimatization in field trials. No conservation strategies on these legumes are reported by earlier workers. In the present study efforts made to initiate the Ex-situ conservation of these plants revealed that establishment of seedling orchards are the base means of multiplication and conservation than any vegetative means.
Inhaltsverzeichnis (Table of Contents)
- Chapter I - Introduction
- 1.1 Importance of Medicinal Plants
- 1.2 Use of plants against microbial infection
- 1.3 Use of plants against free radical damage
- 1.4 The use of plants against mycobacterial infection
- 1.5 Indian Medicinal Legumes
- Chapter II - Review of Literature
- 2.1 Introduction
- 2.2 High performance liquid chromatography
- 2.3 Phytochemical screening assay
- 2.4 Fourier-transform infrared spectroscopy (FTIR)
- 2.5 Phytochemical Screening and Bioactivity of Medicinal Plants
- 2.6 The need for the conservation of medicinal plants
- Chapter III – Methods Used in Isolation and Identification of Bioactive Compounds from Legumes
- Chapter IV - Results and Discussion
- Chapter V - Summary and Conclusion
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This work aims to investigate the bioactivity of Indian legumes and explore methods for their conservation and sustainable utilization. The research focuses on identifying bioactive compounds from these legumes and evaluating their potential in treating various ailments.
- Bioactivity of Indian legumes
- Isolation and identification of bioactive compounds
- Antimicrobial and antioxidant properties of legumes
- Conservation strategies for sustainable utilization
- Application of various analytical techniques (HPLC, FTIR, NMR, etc.)
Zusammenfassung der Kapitel (Chapter Summaries)
Chapter I - Introduction: This chapter establishes the importance of medicinal plants in human health, highlighting their historical and contemporary significance in treating various ailments. It emphasizes the role of plants as a primary healthcare source globally, particularly in regions where traditional medicine is prevalent. The chapter then focuses on Indian medicinal legumes, setting the stage for the research on their bioactivity and conservation.
Chapter II - Review of Literature: This chapter provides a comprehensive overview of existing research on the phytochemical screening, bioactivity, and conservation of medicinal plants. It discusses various analytical techniques used in the identification and characterization of bioactive compounds, such as High Performance Liquid Chromatography (HPLC) and Fourier-Transform Infrared Spectroscopy (FTIR). The chapter also examines the crucial need for the conservation of these valuable plant resources to ensure their sustainable use.
Chapter III – Methods Used in Isolation and Identification of Bioactive Compounds from Legumes: This chapter details the methodology employed in the study. It outlines the steps involved in plant collection, extraction, and the various bioassays used to evaluate the antimicrobial, anti-tubercular, and antioxidant activities of the selected legumes. The chapter also describes the chromatographic and spectroscopic techniques (TLC, HPLC, NMR, FTIR, LC-MS) used for the isolation, purification, and characterization of bioactive compounds. Specific methods like maceration, Soxhlet extraction, and different bioactivity assays (agar well diffusion, disc diffusion, etc.) are explained in detail.
Chapter IV - Results and Discussion: This chapter presents the findings of the research, integrating ethnobotanical data with experimental results. It discusses the phytochemical screening of the selected legumes, the results of the various bioassays (antimicrobial, anti-tubercular, and antioxidant), and the characterization of isolated compounds using various analytical techniques. The chapter analyzes the relationship between the chemical composition and the observed bioactivities, providing an interpretation of the results and their implications.
Schlüsselwörter (Keywords)
Indian legumes, bioactivity, phytochemicals, antimicrobial activity, anti-tubercular activity, antioxidant activity, HPLC, FTIR, NMR, LC-MS, conservation, sustainable utilization, traditional medicine.
Frequently Asked Questions: A Comprehensive Language Preview of Indian Medicinal Legumes
What is the overall focus of this research?
This research investigates the bioactivity of Indian legumes, exploring methods for their conservation and sustainable utilization. It focuses on identifying bioactive compounds from these legumes and evaluating their potential in treating various ailments, including microbial and mycobacterial infections.
What are the key themes explored in this work?
Key themes include the bioactivity of Indian legumes, isolation and identification of bioactive compounds, antimicrobial and antioxidant properties of legumes, conservation strategies for sustainable utilization, and the application of various analytical techniques (HPLC, FTIR, NMR, etc.).
What chapters are included in this work, and what is their content?
The work is structured into five chapters: Chapter I (Introduction) sets the context, emphasizing the importance of medicinal plants and focusing on Indian legumes. Chapter II (Review of Literature) provides a background on relevant research, analytical techniques, and the need for conservation. Chapter III details the methods used for isolating and identifying bioactive compounds. Chapter IV presents the results and discussion of the findings, correlating ethnobotanical data with experimental results. Finally, Chapter V summarizes the conclusions.
What specific analytical techniques are employed in this study?
The study utilizes various analytical techniques, including High Performance Liquid Chromatography (HPLC), Fourier-Transform Infrared Spectroscopy (FTIR), Nuclear Magnetic Resonance (NMR), and Liquid Chromatography-Mass Spectrometry (LC-MS) for the identification and characterization of bioactive compounds. Additional techniques like Thin Layer Chromatography (TLC) and different bioactivity assays (agar well diffusion, disc diffusion, etc.) are also employed.
What types of bioactivity are assessed in the Indian legumes?
The research assesses the antimicrobial, anti-tubercular (against mycobacterial infection), and antioxidant activities of the selected Indian legumes. This involves evaluating the effectiveness of the extracted compounds in inhibiting the growth of various microorganisms and neutralizing free radicals.
What is the significance of the conservation aspect of this research?
The research highlights the critical need for the conservation of medicinal plants, specifically Indian legumes, to ensure their sustainable utilization. It emphasizes the importance of preserving these valuable resources for future generations and preventing their depletion due to overharvesting.
What are the key findings of the research (in summary)?
The research presents the phytochemical screening of the selected legumes, the results of various bioassays (antimicrobial, anti-tubercular, and antioxidant), and the characterization of isolated compounds using analytical techniques. The findings reveal a correlation between the chemical composition and the observed bioactivities of the legumes. Specific details regarding these findings are provided in Chapter IV.
What are the keywords associated with this research?
Key words include Indian legumes, bioactivity, phytochemicals, antimicrobial activity, anti-tubercular activity, antioxidant activity, HPLC, FTIR, NMR, LC-MS, conservation, sustainable utilization, and traditional medicine.
- Quote paper
- Dr. Komal Kumar Javarappa (Author), Dr. A.G. Devi Prasad (Author), 2019, Bioactivity of Indian Legumes and their Conservation for Sustainable Utilization, Munich, GRIN Verlag, https://www.hausarbeiten.de/document/496138
