Objective: To investigate the effect of (i) seed extracts of Pongamia pinnata, Pyrus pyrifolia, and Manilkara hexandra, (ii) bacterial pigment prodigiosin, and (iii) three organic solvents (ethanol, methanol, and DMSO), on quorum sensing (QS) in Chromobacterium violaceum.
Methods: C. violaceum was challenged with plant extracts prepared by microwave assisted extraction (MAE) method, prodigiosin, and organic solvents. Effect of these test substances on C. violaceum growth, and quorum sensing regulated pigment (violacein) production was studied by broth dilution assay. High performance liquid chromatography (HPLC) was also applied to generate chromatographic fingerprint of the active extracts. Effect of sub-MIC level of the antibiotic streptomycin on quorum sensing regulated pigment production was also studied.
Results: P. pinnata seed extracts and prodigiosin were found to possess anti-QS, and M. hexandra and P. pyrifolia seed extracts to possess QS-enhancing effect in C. violaceum. DMSO was found to enhance violacein production, whereas ethanol and methanol reduced violacein production in C. violaceum. Streptomycin at sub-MIC level was able to significantly arrest QS-regulated pigment production in C. violaceum and S. marcescens.
Conclusion: Prodigiosin and the seed extracts used in this study could affect quorum sensing in C. violaceum to a notable extent. Results of this study also emphasizes the importance of inclusion of appropriate solvent controls (negative controls) in bioassays designed for screening of antimicrobial and/or anti-QS compounds. Antipathogenic potential of low concentrations of streptomycin was also demonstrated.
Inhaltsverzeichnis (Table of Contents)
- INTRODUCTION
- PLANT MATERIALS
- Pongamia pinnata
- A. Tree
- B. seeds
Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)
This research paper explores the potential of natural products, particularly from plants, as sources of inhibitors of bacterial quorum-sensing (QS). The study aims to identify and characterize natural compounds that can disrupt the QS communication system in bacteria, thereby preventing the expression of virulence factors and potentially mitigating bacterial infections.
- The use of natural products as a source for new antimicrobial drugs
- The role of quorum-sensing in bacterial virulence
- The identification and characterization of natural quorum-sensing inhibitors
- The potential of specific plants, such as Pongamia pinnata, as sources of QSIs
- The potential applications of QSIs in the fight against bacterial infections.
Zusammenfassung der Kapitel (Chapter Summaries)
- INTRODUCTION: This section provides a general overview of the importance of natural products as a source for new antimicrobial drugs. It discusses the urgent need to combat new and re-emerging infectious diseases and the limitations of existing antimicrobial treatments. The chapter introduces the concept of quorum-sensing (QS) as a key mechanism of bacterial virulence and explores the potential of QS inhibitors (QSIs) as a novel approach to controlling bacterial infections.
- PLANT MATERIALS: This section delves into the specific plant materials chosen for the research, focusing on Pongamia pinnata. It describes the plant's botanical characteristics, its traditional medicinal uses, and its potential as a source of bioactive compounds.
- Pongamia pinnata: This section provides detailed information about the specific plant species, Pongamia pinnata. It includes descriptions of the tree and its seeds, highlighting their potential for medicinal and commercial applications.
Schlüsselwörter (Keywords)
This research paper focuses on natural products, bacterial quorum-sensing, quorum-sensing inhibitors, antimicrobial agents, plant-based medicines, Pongamia pinnata, and medicinal plants.
- Quote paper
- Assistant Professor Vijay Kothari (Author), Haren Gosai (Author), Shreya Raval (Author), Vimla Chaudhary (Author), 2014, Natural products as potential sources of inhibitors of bacterial quorum-sensing, Munich, GRIN Verlag, https://www.hausarbeiten.de/document/286836
