Polyherbal formulations available with a wide range of indications like hepatoprotective , appetite and growth promoters, gastrointestinal and hepatic regulator, as treatment for hepatic dysfunction, for hepatic regeneration as well as liver stimulant and tonic. Despite the widespread use, there is a lack of scientific evidence on their efficacy and safety. This study was designed to evaluate the effect of Polyherbal formulation Kamialri on oxidative stress and associated damages in carbon tetrachloride induced toxicity in Chang liver cells. The study is mainly focused on the evaluation of its antioxidant potential, invitro steatosis and intracellular localisation of reduced glutathione by Monochlorobimane(MCB) staining. Carbon tetrachloride induced toxicity in chang liver cells was well manifested by significant decrease in cell viability, enhanced lipid peroxidation leading to tissue damage,decreased SOD, glutathione, glutathione peroxidase and glutathione S- transferase and increased deposition of fatty acid . MTT study revealed that Kamilari has got cytoprotective activity. It has got the excellent free radical scavenging properties which was proven by various antioxidant activities (LP, SOD, GSH, GPX, and GST). Inhibitory effect of Kamilari on fatty acid deposition was proven by invitro steatosis studies which were conducted on palmitic acid treated chang cell line.Based on the above studies it is concluded that Kamilari has cytoprotective and antioxidant activity, and found to be effective in steatosis.
Table of Contents
1. Introduction
2. Materials & Methods
2.1 Invitro protective effect of Kamilari in Ccl4 treated Chang liver cells
2.2 Lipid peroxidation
2.3 Estimation of SOD activity
2.4 Estimation of reduced glutathione
2.5 Assay of Glutathione peroxidase
2.6 Glutathione -s-transferase
2.7 Oil Red O
3. Results
3.1 Cytoprotective effect of Kamilari in Chang liver cells.
3.2 Antioxidant enzymes and markers
4. Discussion
5. Conclusion
Research Objectives & Topics
The primary objective of this study is to evaluate the cytoprotective and antioxidant potential of the polyherbal formulation 'Kamilari' against carbon tetrachloride (Ccl4) induced oxidative stress and its therapeutic efficacy in hepatic steatosis within Chang liver cell lines.
- Evaluation of hepatoprotective activity against Ccl4 toxicity.
- Assessment of antioxidant potential through various enzyme markers.
- In vitro induction and management of hepatic steatosis.
- Intracellular localization of reduced glutathione using fluorescence staining.
- Dose-dependent efficacy analysis of Kamilari.
Excerpt from the Book
1. Introduction
Liver is an important organ that plays an essential role in regulating various physiological processes in the body. It is involved in several vital functions, such as metabolism, secretion and storage. In vitro liver systems represent a better experimental approach to screen potential hepatotoxic compounds . Liver cell lines are characterized by unlimited subcultivation and cell availability in large number1 . The hepatotoxin used was carbon tetrachloride because it has long been known as a model toxicant and has been the focus of many in vitro and in vivo toxicological studies2 . The liver is the major target organ of carbon tetra chloride toxicity owing to its high content of Cytochrome P-4503.
Kamilari is a commercial ayurvedic formulation suggested to be effective in treatment of liver disorder such as jaundice, chronic alcoholism, acute and chronic inflammatory disorders. As per the manufactures information Kamilari consist of Thespesia populinea, Eletteria cardamom, Zingiber officinalis, Glyccyrhiza glabra, Piper longum, Aegle marmelos, Trichosanthes cucumerina, Holostemma adakodein Schulter and Honey.Since large mass of populations used preferable herbal preparation, therefore there is need to be evaluate the proper mechanism underlying the hepatoprotective effect.
Summary of Chapters
1. Introduction: Outlines the physiological role of the liver, the necessity of in vitro screening for hepatotoxins, and the traditional medicinal claims of the polyherbal formulation Kamilari.
2. Materials & Methods: Details the specific experimental protocols, including chemical preparations, cell culture maintenance, and the assays used for antioxidant potential and steatosis induction.
3. Results: Presents the experimental data demonstrating the cytoprotective effects of Kamilari against Ccl4, evidenced by antioxidant enzyme restoration and reduction of lipid droplet accumulation.
4. Discussion: Interprets the findings by contextualizing the role of antioxidants in protecting liver cells and validating the efficacy of Kamilari in alleviating oxidative damage.
5. Conclusion: Summarizes the study's findings, confirming that Kamilari possesses significant cytoprotective and antioxidant activities and is effective in treating steatosis conditions in Chang liver cells.
Keywords
polyherbal formulation, kamilari, steatosis, hepatoprotective activity, carbon tetrachloride, oxidative stress, Chang liver cells, lipid peroxidation, superoxide dismutase, glutathione, glutathione peroxidase, glutathione s-transferase, antioxidant potential, cell viability, ayurvedic medicine.
Frequently Asked Questions
What is the core focus of this research study?
The study investigates the hepatoprotective and antioxidant properties of the ayurvedic polyherbal formulation 'Kamilari' using in vitro Chang liver cell models exposed to toxicity.
What are the primary thematic areas addressed?
Key themes include hepatic toxicity induced by carbon tetrachloride, oxidative damage mechanisms, the role of herbal interventions, and the pathophysiology of fatty liver (steatosis).
What is the main research objective?
The goal is to scientifically validate the traditional claims of Kamilari by evaluating its ability to reduce oxidative stress and inhibit fatty acid accumulation in liver cells.
Which scientific methodology is employed?
The study utilizes in vitro cell culture techniques, employing various biochemical assays like MTT for cell viability, spectrophotometric methods for enzyme markers, and fluorescence staining for glutathione.
What does the main body cover?
It covers the systematic culturing of cells, the induction of damage using Ccl4 and palmitic acid, and a rigorous analysis of enzyme levels and lipid droplets post-treatment with Kamilari.
Which keywords define this work?
Relevant keywords include polyherbal formulation, Kamilari, steatosis, hepatoprotective activity, oxidative stress, and antioxidant enzymes.
How does Kamilari affect glutathione levels in stressed cells?
Kamilari was shown to significantly increase reduced glutathione levels, which were otherwise depleted by Ccl4 exposure, as verified by Monochlorobimane (MCB) fluorescent staining.
Is the effect of Kamilari dose-dependent?
Yes, the results indicate that Kamilari exhibits cytoprotective activity in a dose-dependent manner, showing high efficacy at lower doses.
- Quote paper
- Dr.Sibi P.Ittiyavirah (Author), 2012, Hepatoprotective activity of Kamilari, Munich, GRIN Verlag, https://www.hausarbeiten.de/document/268298
